【摘要】 本研究采用H.E.染色、硫化铅组织化学染色及IgG免疫组织化学染色的方法对0、5、28日龄仔猪小肠进行了组织研究。结果显示:仔猪小肠IgG阳性反应物分布于小肠绒毛柱状细胞的转运泡、柱状细胞基底部、小肠小血管血浆、淋巴管淋巴中,中央乳糜管周围的结缔组织中也有分布。仔猪小肠IgG阳性强度随日龄的增加逐渐下降。在各肠段中,空肠IgG阳性最强。猪小肠酸性磷酸酶的分布规律与IgG相似。仔猪小肠上皮内淋巴细胞数目随日龄增加显著增多,集合淋巴小结由0日龄至28龄逐渐发育成熟。结论:仔猪在出生时通过胞吞作用摄取母源抗体IgG,伴随此过程的还有溶酶体的细胞内消化作用。最终通毛细血管和毛细淋巴管途径进行转运,0~5日龄是仔猪小肠摄取母源抗体IgG的最佳时机,至28日龄,仔猪小肠发生“肠关闭IgG摄取停止。与此同时,小肠淋巴组织逐渐发育成熟,肠道的黏膜免疫屏障趋于完善。更多还原

【Abstract】 The small intestines of piglets aged 0, 5 and 28 days were examined by HE, PbS histoche-mical and IgG immunohistochemical staining. The results were as follows: The IgG immunoreaction positive substance distributed in transport vesicles and fundus of columnar cells in villus, blood plasma of small blood vessels, lymphoplasma and connective tissues around central lacteals. With the development of piglets, the intensity of IgG immunoreaction positive substance decreased. The intensity of IgG immunoreaction positive substance in jejunum was the strongest among 3 parts of the small intestine. The distribution of acid phosphate enzyme was similar to that of IgG immumoreaction positive substance. The number of intra epithelial lymphocytes increased with the development of piglets. Peyer’s patch matured gradually from 0 to 28 days after birth. In conclusion, newborn piglets uptake maternal IgG by endocytosis besides the intracellular digestion of lysosome. And maternal IgG willbe eventually transported by blood capillary and lymphatic capillary. The best time of maternal IgG uptaking is 0 to 5 days after birth. “Gut closure” occurs in 28 days after birth, and maternal IgG uptaking will end then. At the same time, gut-associated lymphoid tissue mature gradually, and immuno-barriers of mucous membrane develop to perfection.更多还原