【摘要】 用高效液相色谱法测定大黄不同炮制品中大黄素的含量。采用Hypersil Gold C18柱(150 mm×4.6 mm,5μm),以甲醇-0.05%磷酸(85∶15)为流动相,流速1.0 mL/m in,检测波长254 nm。大黄素的线性检测范围为4.79~47.9μg/mL,线性良好,R2=1.000;大黄素平均加样回收率为98.56%,RSD为1.30%(n=5)。生大黄及各炮制品中大黄素含量分别为:生大黄0.401%,熟大黄0.669%,酒大黄0.512%,醋大黄0.348%。试验得出如下结论:炮制使大黄中大黄素的含量产生明显变化。更多还原

【Abstract】 An HPLC method to determine the emodin in different processed rhubarb was established with a Hypersil Gold C₁₈（150 mm×4.6 mm,5 μm） column.The mobile phase was composed of methanol and 0.05% H₃PO₄ solution（85∶15,V/V）,the flow rate was 1.0 mL/min,the detection wavelength was 254 nm,and the column temperature was room temperature.The calibration curve was linear in the concentration range of 4.79⁴7.9 μg/mL with correlation coefficient of 1.000. The mean recovery of the method was 98.56%,RSD was（1.30%）（n=5）.The concentrations of emodin in Radix et Rhizoma Rhea and its processed products are as follows,0.401% in Radix et Rhizoma Rhei,0.669% in ripe Radix et Rhizoma Rhei,0.512% in winy Radix et Rhizoma Rhei,and 0.348% in acetic Radix et Rhizoma Rhei,respectively.The results showed that the content of emodin in Radix et Rhizoma Rhei was different from that in the corresponding processed drugs.更多还原