【摘要】 180只1日龄艾维茵肉鸡健雏随机分为3组,分别饲喂对照(每千克日粮含铜11.97 mg)和铜中毒(每千克日粮含铜650 mg,铜中毒组;每千克日粮含铜850 mg,铜中毒组)日粮6周,以流式细胞术和酸性-α-醋酸萘酯酶(ANAE)染色的方法观测外周血T-淋巴细胞的动态变化。2个铜中毒组1~6周龄外周血T-淋巴细胞的ANAE阳性率均显著低于对照组(P<0.05或P<0.01),同时2个铜中毒组间比较也呈显著差异(P<0.05或P<0.01)。与对照组比较,2个铜中毒组2~6周龄CD4+T淋巴细胞数量均显著降低(P<0.05或P<0.01),CD8+T淋巴细胞数量变化不明显(P>0.05),CD4+/CD8+比值降低(P<0.05)。结果表明,铜中毒可抑制T-淋巴细胞的生成,降低其在外周血中的数量。更多还原

【Abstract】 The experiment was conducted to examine the effect of copper toxicity on the peripheral blood T-lymphocyte using the flow cytometry（FCM） and ANAE.180 one-day-old Avian broilers were divided randomly into three groups,and fed diets as follows:（1）Controls（Cu 11.97 mg/kg diet） and（2）copper toxic（Cu 650 mg/kg diet,copper toxic group Ⅰ;Cu 850 mg/kg diet,copper toxic group Ⅱ） for six weeks.The ANAE positive ratios of the peripheral blood T-lymphocytes were much lower in the two copper toxic groups than in control group from 1 to 6 weeks of age（P<0.05 or P<0.01）.Also,there was significant difference between copper toxic groups Ⅰ and Ⅱ at 1,3,5 and 6 weeks of age（P<0.05 or P<0.01）.CD⁺₄ T cell numbers reduced from 2 to 6 weeks of age in both copper toxic group Ⅰ and copper toxic group Ⅱ as compared with those of control group（P<0.05 or P<0.01）.At the same time,there was significant difference between copper toxic groups Ⅰ and Ⅱ at 6 weeks of age（P<0.05）.But the numbers of CD⁺₈ T cell were not varied from 2 to 6 weeks of age in copper toxic groups Ⅰ and Ⅱ in comparison with those of control group（P>0.05）.The CD⁺₄/CD⁺₈ ratio was lower from 2 to 6 weeks of age in copper toxic groups Ⅰ and Ⅱ than in control group.The results showed that copper toxicity could suppress the development of T-lymphocytes and reduce the peripheral blood T-lymphocyte populations.Potential mechanisms underlying these observations are also discussed.更多还原