【摘要】 200只1日龄艾维茵肉鸡随机分为4组,即对照组(每千克日粮含锌100mg)、锌中毒组(每千克日粮含锌1500mg)、锌中毒组(每千克日粮含锌2000mg)、锌中毒组(每千克日粮含锌2500mg),每组均饲喂日粮7周。以流式细胞术和酸性α-醋酸萘酯酶(ANAE)染色法观测外周血T-淋巴细胞的动态变化。结果,3个锌中毒组7周龄时,外周血T-淋巴细胞ANAE阳性率显著低于对照组(P<0.05或P<0.01),同时3个锌中毒组间比较也呈显著差异(P<0.05或P<0.01)。与对照组比较,锌中毒组2至7周龄和锌中毒组6、7周龄CD4+T淋巴细胞数量减少,锌中毒组、组2至4周龄和锌中毒组、组7周龄CD8+T淋巴细胞数量降低,CD4+/CD8+比值3个锌中毒组2、4周龄升高,6、7周龄降低。由此表明,锌中毒可抑制T-淋巴细胞的生成,降低其在外周血中的数量。更多还原

【Abstract】 The experiment was conducted to examine the effect of zinc toxicity on the peripheral blood T-lymphocyte by the method of flow cytometry（FCM） and ANAE.200 one-day-old Avian broilers were divided into four groups,and fed with the diets as follows:①control group（Zn 100 mg/kg diet） and ② zinc toxic groups（Zn 1 500 mg/kg diet,zinc toxic group Ⅰ;Zn 2 000 mg/kg diet,zinc toxic group Ⅱ;Zn 2 500 mg/kg diet,zinc toxic group Ⅲ） for seven weeks.The ANAE positive ratios of the peripheral blood T-lymphocytes were much lower in the three zinc toxic groups than that in control group at 7 weeks of age.CD₄+ T cell numbers were reduced from 2 to 7 weekds of age in zinc toxic group Ⅲ and from 6 to 7 weeks of age in zinc toxic group Ⅱ,as compared with that of control group.The numbers of CD₈+ T cell decreased at 2 and 4 weeks of age in zinc toxic groups Ⅱ and Ⅲ,and at 7 weeks of age in zinc grups Ⅰ and Ⅲ.CD₄+/CD₈+ ratio was higher at 2 and 4 weeks age,and lower at 6 and 7 weeks of age in zinc toxic groups Ⅰ,Ⅱ and Ⅲ than in control group.The results showed that zinc toxicity would suppress the development of T-lymphocytes and reduce the peripheral blood T-lymphocyte populations.Potential mechanism underlying these observations are also discussed.更多还原