【摘要】 【目的】建立能反映肝癌肝移植特点的肝癌复发动物模型。【方法】选用Wistar大鼠,实验组大鼠给予肝移植术后常规联合免疫抑制方案(MP+CSA),对照组仅同法使用生理盐水,两组均接受经门静脉系统接种walker-256肿瘤细胞株手术,观察60d,死后剖腹探查取病理检查。【结果】实验组手术时间为(22.6±0.6)min,对照组为(22.1±0.7)min,两组间差异无统计学意义(P>0.05);实验组生存时间为(14.8±2.3)d,对照组为(40.9±5.3)d,两组间差异有统计学意义(P<0.05)。实验组大鼠体质量减轻量(53.5±2.6)g,较对照组体质量减轻量(34.6±5.8)g差异有统计学意义(P<0.05);实验组大鼠肿瘤复发率为95%(19/20),对照组为40%(8/20),实验组大鼠肿瘤的复发率明显高于对照组大鼠(P<0.05)。【结论】该模型能够有效模拟肝癌肝移植术后肿瘤复发的主要特征,可以作为研究肝癌肝移植术后肿瘤复发的理想大鼠模型。更多还原

【Abstract】 Objective To establish a new animal model for investigating the recrudescent mechanism after liver transplantation for hepatocellular cancer.Methods The 40 wistar rats were chosen and randomly divided into 2 groups,immunosuppression group and control group.The walker-256 cell line was injected via portal venous in both groups.Immunosuppression group received the combined immunosuppression regime(MP+CSA).Control group was applied NS simultaneity.Pathologic anatomic characters of the livers were collected by exploratory laparotomy after death or 60 days after the above treatment in both groups.Results The operation time of immunosuppression group was(22.6±0.6)min,while control group(22.1±0.7)min.No significant difference was found between them(P >0.05).The weight loss in immunosuppression group was higher than control group,which was(53.5±2.6)g and(34.6±5.8)g respectively(P< 0.05).Survival time were(14.8±2.3)days in immunosuppression group,shorter than control group(40.9±5.3)days(P< 0.05).The percentage of recrudescence rate was 95%(19/20)in immunosuppression group,higher than control group,40%(8/20)(P< 0.05).Conclusion This model is an optimal clinical model to mimic the natural recrudescence characters of hepatocellular carcinoma in human body after liver transplantation.更多还原