【摘要】 【目的】以逆转录(RT)和实时定量聚合酶链式反应(real-timeqPCR)方法检测和分析非酒精性脂肪肝(NAFLD)大鼠模型的药物干预效果。【方法】以高脂饲料饲喂雄性Wistar大鼠12周诱发NAFLD,然后转以基础饲料饲养,并分别进行盐酸二甲双胍干预与蒸馏水灌喂对照,每组8只,为期4周。从大鼠脂肪组织提取总RNA并逆转录成为cDNA,通过real-timeqPCR检测肿瘤坏死因子α(TNF-α)mRNA的相对水平,以t检验分析两组间数据的差异。【结果】干预组大鼠脂肪组织的TNF–αmRNA相对水平平均值(0.063±0.006)比对照组(0.126±0.015)下降,其差异显著(t=4.662,P=0.001)。【结论】盐酸二甲双胍可以显著地降低NAFLD大鼠脂肪组织TNF-α基因的表达水平,能够调节肝脏脂肪代谢和改善肝细胞脂肪变性。更多还原

【Abstract】 [Objective] To detect and analyze the effect of medical intervention for the rat model of non-alcoholic fatty liver disease (NAFLD) using the reverse transcription (RT) and real-time quantitative polymerase chain reaction (Real-time qPCR). [Methods] The male Wistar rats were fed with high fatty chow for 12 weeks to induce NAFLD. And then, two groups of these rats were fed with the low fatty chow, and separately intervened by metformin hydrochloride or controlled by distill water for 4 weeks. Each group consisted of 8 rats. The total RNA of adipose tissue was extracted and reversely translated into cDNA. The relative level of tumor necrosis factor α (TNF-α) mRNA was detected in the real-time qPCR. The data difference between two groups was analyzed by t-test. [Results] The relative level of TNF-α mRNA in adipose tissue of intervenient group (0.063±0.006) was decreased than that of control group (0.126±0.015). Their difference was significant (t=4.662,P=0.001). [Conclusion] Metformin hydrochloride can significantly decrease the expression level of the TNF,α gene in adipose tissue of NAFLD rats. It can adjust the adipose metabolism of liver and improve the steatosis in liver cell.更多还原