【摘要】 在摇瓶发酵条件下,采用Plackett-Burman设计法和响应面分析法对枯草芽孢杆菌B908菌株的培养基配方进行优化,确定了影响B908菌株发酵液含菌量的3个重要因子依次为玉米粉、碳酸钙和豆饼粉;对这3个因子进行爬坡路径试验,通过响应面分析法确定了主要影响因子的最佳条件。优化后的培养基配方使摇瓶发酵液每毫升的含菌量从0.6×109个/ml提高到1.18×1010个/ml。更多还原

【Abstract】 Plackett-Burman design and response surface analysis were used to optimize the fermentation medium of Bacillus subtilis B908.Based on the results of Plackett-Burman design,important factors affecting the yield of Bacillus subtilis B908 were corn flour,bean flour and CaCO₃.Then,the three factors were further optimized using response surface analysis.The optimized fermentation medium allowed the production to be increased from 6.6×10⁹spores ml^-1 to 1.18×10¹⁰spores ml^-1.更多还原