【摘要】 瑞香狼毒素1,5-二苯基-3-羟基-1-戊酮,是本室从杀虫植物瑞香狼毒中分离得到的一个高活性化合物。本文研究了该活性成分对菜粉蝶幼虫的拒食作用和对乙酰胆碱酯酶(AChE)活性的影响。采用浸渍叶碟饲喂法测得:在48h时瑞香狼毒素对3龄幼虫的非选择性和选择性拒食作用的拒食中浓度(AFC50)分别为15.1mg/L和16.9mg/L。采用点滴法测得瑞香狼毒素对5龄幼虫的拒食作用的拒食中量(AFD50)为0.565μg/头。瑞香狼毒素对AChE活性的抵制率高达78.5%,表明该活性成分可显著抑制AChE活性。更多还原

【Abstract】 A high bioactive compound 1,5-diphenyl-3-hydroxyl-1-pentanone was isolated from the extract of the roots of Stellera chamaejasme L.The antifeedant activity of the compoud was tested against larvae of Pieris rapae by leaf dipping method and topical application method.The results showed that the AFC₅₀ of non-choice and choice antifeeding effects in leaf-dipping test against third instar larvae were 15.1mg/L and 16.9mg/L respectively in 48 hours,and the AFD₅₀ of non-choice antifeeding effect by topical application method against fifth instar larvae was 0.565 μg/larva.The microplate assay method was used to determine the activity of acetylcholinesterase.The results showed that 1,5-diphenyl-3-hydroxyl-1-pentanone could significantly inhibit acetylcholine esterase.更多还原