【摘要】 从烟草根际分离筛选到一株可产生抗烟草赤星病菌物质的枯草芽孢杆菌菌株B75。在以NB培养液进行试验时发现,B75在对数生长的中期开始产生抗菌物质,在生长的衰退期前存在一个产生抗菌物质的高峰期;在25~50℃、pH4.0~8.5范围内均可产生抗菌物质,其中30℃和pH6.0的培养条件下最适。不同的装液量试验结果表明,在三角瓶装液量为100ml/500ml时,是B75菌株菌体生长及产生抗菌物质的最适宜量。不同的碳、氮源试验结果表明,甘露醇、酵母膏最有利于B75菌株菌体生长,且产生的抗菌物质抑菌活性较强。更多还原

【Abstract】 An antagonistic strain B75,isolated from tobacco rhizosphere soil,began to produce antagonistic substance(AS)at logarithmic growth phase,and reached a peak before its growth stopped in NB medium.In 50-250 ml NB medium of 500 ml flask with pH 4.0-8.5,B75 could produce AS at 25-50℃,while 100 ml medium in 500 ml flask,pH 6.0 and 30℃ were the best conditions.Among 17 carbon and 6 nitrogen sources tested,mannitol and yeast extract were demonstrated to be more suitable for production of B75 AS.更多还原