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反转录聚合酶链反应方法诊断肠道小RNA病毒性脑膜炎

Diagnosis of Picornavirus Group Meningitis Using Reverse Transcription-Polymerase Chain Reaction

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【作者】 关慧臻杨桂梅薛承岩朱长林来锦白海洋

【Author】 GUAN Hui-zhen, YANG Gui-mei, XUE Cheng-yan, ZHU Chang-lin, LAI Jin, BAI Hai-yang. (Affiliated Hospital of Chengde Medical College, Chengde 067000, China)

【机构】 承德医学院附属医院承德医学院附属医院 河北承德市067000河北承德市067000

【摘要】 目的评估RT-PCR方法诊断肠道小RNA病毒性脑膜炎的应用价值。方法对象包括脑膜炎组104例和对照组39例,检测脑脊液中肠道小RNA病毒应用RT-PCR方法。结果脑膜炎组的肠道小RNA病毒阳性率为41.3%(43/104),对照组未测出肠道小RNA病毒阳性结果。结论肠道小RNA病毒是导致脑膜炎的重要病原体,RT-PCR方法诊断肠道小RNA病毒性脑膜炎具有较高的应用价值。

【Abstract】 Objective To assess the value on the diagnosis of picornavirous group meningitis by RT-PCR. Methods The objects consist of the patients with meningitis (104 cases) and the patients with non- meningitis disease(39 cases). Picornavirous group was detected in cerebrospinal fluid (CSF) with reverse transcription-polymerase chain reaction (RT-PCR). Results The positive rate was 41.3% (43/104) in meningitis group. The control cases were not any positive results of picornavirous group.Conclusions The picornavirous group is the important pathogens, which cause meningitis. RT-PCR has higher value in the diagnosis of picornavirous group meningitis.

  • 【文献出处】 中国实用医药 ,China Practical Medical , 编辑部邮箱 ,2006年06期
  • 【分类号】R450;R512.3
  • 【被引频次】1
  • 【下载频次】70
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