【摘要】 目的研究耐药性癫患者脑细胞线粒体mMDH、NDUFC2表达,探讨其在耐药性癫癎形成中的作用。方法分别提取48例耐药性癫癎患者、8例非耐药性癫患者及8例对照组脑组织标本的总RNA后,用基因芯片对其进行扫描,随后用荧光定量PCR技术进行验证。结果发现与线粒体功能有关的基因mMDH、NDUFC2在耐药性癫癎中显著下调,荧光定量RT—PCR验证结果与基因芯片一致。结论脑细胞线粒体基因mMDH、NDUFC2表达异常可能通过能量代谢及神经元坏死参与了耐药性癫癎的形成。更多还原

【Abstract】 Objective To investigate the expression of mMDH、NDUFC2 in the brain tissue from the patients withpharmacoresistanee epilepsy and explore the possible functions of energy metabolism in the pathogenesis of pharmacoresis-tance epilepsy.Methods After total RNA was extracted from brain tissue samples of 48 pharmacoresistant epilepsies,8drug-sensitive epilepsies and 8 control eases,respectively,we examined the gene expression by fluorescent quantitative re-al-time reverse transcription polymerase chain reaction after a comparision of cDNA micromTays.Results A significantdownregulation of mMDH and NDUFC2 which was related with the function of mitochondrion was observed,the real-timeRT-PCR suggested that the relative expression level of the mMDH in pharmacoresistance epilepsy was 27.9% of that indrug-sensitive group.Conclusion The abnormal expressions of mitochondrial genes mMDH、NDUFC2 in brain tissue areinvolved in the pathogenesis of pharmacoresistance epilepsy by energy metabolism and apoptotic death of neurons.更多还原