【摘要】 目的:探讨补阳还五汤及其有效部位生物碱和苷对大鼠动脉血栓形成及血浆血栓素A2(TXA2)和前列环素(PGI2)的影响。方法:对颈总动脉血栓模型大鼠分别给予补阳还五汤原方、生物碱、苷、低分子肝素和抵克利得,比较各组动脉血栓重量和血浆TXB26、-keto-PGF1α的变化。结果:模型组可形成闭塞性血栓,TXB2升高(P<0.05),6-kcto-PGF1α降低(P<0.05)。各药均可使血栓重量减轻。原方可使6-keto-PGF1α升高(P<0.05),而对TXB2无显著影响。生物碱可降低TXB2(P<0.05),升高6-keto-PGF1α(P<0.05)。苷、低分子肝素和抵克利得对TXB2和6-keto-PGF1α均无显著影响。结论:补阳还五汤原方、生物碱、苷、低分子肝素和抵克利得均有抗血栓作用。原方的作用与促进PGI2合成有关;生物碱的作用与抑制TXA2合成、促进PGI2合成有关。而苷、低分子肝素和抵克利得不是通过调节TXA2和PGI2合成发挥作用的。更多还原

【Abstract】 Objective:To explore effect of Buyang Huanwu Decoction（BHD） and its active fractions such as alkaloid and glycoside extracted from BHD on thromboxane（TXA₂） and prostacyclin（PGI₂） of arterial thrombosis model in rats.Methods:Carotid arterial thrombosis model in rats was induced by topical ferric chloride.The model rats were administered with BHD,alkaloid,glycoside,low molecular weight heparin（LMWH） and ticlopidine respectively.Weight of thrombus and the levels of TXB₂ and 6-keto-PGF_1α in plasma in each group were compared.Results:Occlusive thrombus was induced,the levels of TXB₂ were increased markedly（P<0.05）,the levels of 6-ketoPGF_1α were lower remarkably in the model group（P<0.05）.Weight of thrombus in BHD,alkaloid,LMWH and ticlopidine group was significantly lower than that of the model group（P<0.05）.BHD could increase the levels of 6-ketoPGF_1α（P<0.05）,but it did not influence the levels of TXB₂.Alkaloid could decrease the levels of TXB₂ and increase the levels of 6-keto-PGF_1α significantly（P<0.05）.Glycocide,LMWH and ticlopidine had no influence on the levels of TXB₂ and 6-keto-PGF_1α.Conclusions:Antithrombotic actions of BHD,alkaloid,glycoside,LMWH and ticlopidine were observed.Antithrombotic actions of BHD may be related to accelerating synthesis of PGI₂.Antithrombotic actions of alkaloid may be associated to inhibiting production of TXA₂ and increasing synthesis of PGI₂.Antithrombotic actions of glycoside,LMWH and ticlopidine was not based on its modulation on TXA₂ and PGI₂,it may be concerned with other aspects.更多还原