【摘要】 目的探讨阿胶对哮喘大鼠Th1/Th2细胞因子的影响及其防治哮喘的作用机制。方法60只Wistar大鼠随机分为生理盐水(NS)对照组(A),低剂量阿胶组(B),中剂量阿胶组(C),高剂量阿胶组(D),斯奇康组(E),哮喘对照组(F)。用酶联免疫吸附试验(ELISA)检测血液中白介素-4(IL-4)、γ干扰素(IFN-γ)水平的变化。HE染色观察肺组织病理学变化。结果与NS对照组比较,哮喘模型组血液中IFN-γ水平显著降低(P<0.01)。与哮喘模型组比较,阿胶低、中剂量组和斯奇康组IL-4水平显著降低(P<0.0 1),NS对照组、阿胶低、中剂量组和斯奇康组人鼠肺组织嗜酸性细胞浸润程度也明显减轻。结论哮喘大鼠存在Th2型细胞优势反应,而阿胶可能具有抑制哮喘Th2细胞优势反应的作用,从而调节Th1/Th2型细胞因子平衡,同时,可减轻哮喘大鼠肺组织嗜酸性细胞炎症反应。更多还原

【Abstract】 Objective:To study the effects of Ejiao on Th1/Th2 cytokines and the mechanism of which preventing and curing bronchial asthma.Methods:60 wistar rats were randomly divided into 6 groups.Group A was normal group which were administered with NS.Group B,C and D were administered with low,moderate and high dose Ejiao.Group E were administered with Polysaceharidc.Group F were asthma group.1L-4 and IFN-γ in serum were detected by enzyme-linked immunosorbent assay(ELISA).Results:Compared with normal group,the concentration of IFN-γ in group F was decreased significantly.compared with asthma group,the concentration of IL-4 of low and moderate dose Ejiao groups and Polysaceharidc group were decreased significantly(P<0.05),and there was less eosinophils in the lungs of group A、B、C、E than that in group F.Conclusion:Ejiao could regulate the ratio of Th1/Th2 by decreasing Th2 subset instead of increasing Th1 subset.On the other hand,it could inhibit eosinophils to move from peripheral blood to the lungs of asthma rats.更多还原