【摘要】 目的:探讨CK7、8、19,白蛋白,甲胎蛋白AFP和CD34在慢性重型肝炎(CSH)患者肝组织内的表达;分析肝内胆管增生和肝再生的关系。方法:12例CSH肝组织行HE和嗜银染色;用SP免疫组织化学染色检测CK7、8、19,白蛋白、AFP和CD34表达。结果:死亡2例诊断为慢性重型肝炎;10例恢复期患者病理诊断为早期肝硬化。肝组织内胆管明显增生。肝组织内典型胆管增生细胞CK7、19强阳性;非典型胆管增生细胞CK8强阳性,CK7、19呈弱阳性,部分同时表达CK19和白蛋白,其细胞形态和表型符合肝卵圆细胞特征。结论:慢性重型肝炎患者肝组织内部分非典型增生胆管细胞可能来源于肝卵圆细胞;慢性重型肝炎患者肝细胞增生可能是干细胞参与的再生过程。更多还原

【Abstract】 Objective:To investigate the correlation between the bile-duct proliferation and the expression of cytokeratins,Albumin,alpha-fetoprotein(AFP),CD34 in liver specimens of patients with chronic severe hepatitis(CSH),then study the correlation between duct proliferation and hepatocytes regeneration in CSH patients.Methods:12 liver specimens of CSH patients were cxonined with HE and silver staining;then the expressions of CK19,Albumin,AFP,CD34 in these specimens were assessed with immunohistochemical technique SP.Results:Biliasg ductular proliferation increased in CSH liver specimens significantly.It shooed stronger positive of CK7,19 staining in typical hieong ductule proliferationand less in some atypical ones,which revealed stronger positive of CK8.In atypical vieiang ductular proliferation,some cells not only had the ovoid nucleus and scant cytoplasm,but co-expression of CK19 and albumin were observed in them,like the oval cell in shape and immunohistochemical characteristic.Conclusion:We conjectured that some cells in atypical ductular proliferation must have derived from oval cell or had the oval cells in origin and hepatocytes regeneration might have been derived from the liver stem cell in CSH patients.更多还原