【摘要】 从犬脑组织中提取总RNA,利用设计的一对引物N1/N2,扩增出1513bp的目的片段,通过SphI和SalI两个酶对扩增产物进行酶切,得到了457bp、833bp、233bp大小的3个预期片段,从而能在8 h内快速做出正确的诊断。试验表明,用该技术检测狂犬病病毒灵敏度高、特异性强,适合于实验室应用。更多还原

【Abstract】 A new method by using PCR-RFLP for the rapid diagnosis of rabies was established,in which a 1513bp target fragment was amplified from brain tissues of dogs by PCR with a pair of the designed primers N1/N2,and then digested by SphI and SalI,thus producing the predicted 3 fragments with 457 bp,833 bp and 233 bp in size.These products were subjected to electrophoresis on 1% agarose gel.By using the method to analyze the restriction fragment length polymorphism,a correct diagnosis for babies can be made within 8 hours.Owing to its higher sensitivity and specificity,this new method by using PCR-RFLP for rapid diagnosis of rabies is very suitable for practical use in laboratories.更多还原