【摘要】 目的本研究在自制T-2毒素酶标半抗原的基础上,结合市售抗T-2毒素单克隆抗体,建立了检测谷物中T-2毒素的直接竞争性酶联免疫吸附测定法(ELISA)。方法粮食样品经70%甲醇-水溶液提取,用滤纸过滤并稀释后,再用0.45μm滤器过滤净化,该滤液用于ELISA检测。结果本方法最低检出量为0.125ng/ml,添加70~280ng/g T-2毒素的大米样品和面粉样品的平均回收率分别为85~117.5%和98.1~102.5%。结论结果表明,该方法简化了粮样提取步骤,可用于谷物及加工产品中T-2毒素的检测。更多还原

【Abstract】 A monoclonal antibody based-direct competitive enzyme linked immunoabsorbent assay(ELISA) was developed for the quantitative determination of T-2 toxin in cereals in the present study,in which 70% of methanol-water solution was used to extract the cereal samples;The extracts filtered through Whatman No.4 filter paper and diluted,were clarified by passing through the filter with 0.45 μm pores,and this purified extract was used in the detection of T-2 toxin by ELISA.It was found that the minimal amount of toxin to be detected by this method was 0.125 ng/ml,and the recovery rates of the rice and flour samples spiked with 70-280 ng of T-2 toxin per gram were 92.3%-112.7% and 98.8%-102.5% respectively.It is evident that this method of testing is proved to be suitable for the screening of T-2 toxin in cereals.更多还原