【摘要】 目的:建立大鼠脑组织蛋白质双向电泳技术。方法:利用不同体积的裂解液提取大鼠脑组织中的蛋白质,并通过不同的蛋白质上样量(1mg,2mg,3mg),进行双向电泳,考马斯亮蓝染色,图谱分析。结果:在等电点3～10,分子量6.5～200ku范围内分离得到蛋白质斑点为,1mg蛋白质上样量为546个蛋白质斑点,2m g为780个,3m g为805个斑点。2mg蛋白质上样量的双向电泳图谱更清晰,分离更好。结论:成功建立了大鼠脑组织蛋白质的双向电泳技术。更多还原

【Abstract】 Objective:To establish a two-dimensional electrophoresis technology on brain tissue protein of rat.Methods:Lysis buffer of different volume was taken to extract brain tissue proteins of rat,and different protein quantities(1mg,2mg,3mg) were taken to establish a two-dimensional electrophoresis.Coomassie brilliant blue was applied to stain protein,and patterns were analyzed.Results:With a molecular mass between 6.5~200ku and isoelectric points(pI) from 3~10,1mg proteins obtained 546 protein spots,2mg 780,and 3mg 805.Pattern of 2mg protein was the best.Conclusion:A two-dimensional electrophoresis technology on brain tissue protein of rat has been established successfully.更多还原