【摘要】 目的:观察人参皂甙Rg1对实验性氧化损伤兔晶状体上皮细胞(LEC)凋亡相关基因Bcl-2和Bax表达的影响。方法:复制兔晶状体氧化损伤模型,同时加入终浓度为10μmol/L的人参皂甙Rg1共同孵育24h,取晶体前囊膜,采取免疫组化法,检测凋亡相关基因Bcl-2和Bax的蛋白表达。结果:Bcl-2和Bax在正常兔晶体上皮细胞中均有表达,Bcl-2的表达远较Bax强;过氧化氢组Bcl-2的表达显著下调,Bax表达显著上调,Bcl-2和Bax比率下降;Rg1可明显上调Bcl-2的表达,下调Bax的表达,Bcl-2/Bax比率上升,Bcl-2改变幅度与牛磺酸比较有显著性差异(P<0.05)。结论:氧化损伤诱导LEC凋亡和Rg1抑制LEC凋亡的分子机制可能与凋亡相关基因Bcl-2和Bax表达有关。更多还原

【Abstract】 Objective:To investigate the effect of Rg1 on the expression of apoptosis related genes of LEC induced by experimental oxidative injuries.Methods:All fresh transparent lenses of rabbit except those in the control group were bathed in a culture medium with 300μmol/L hydrogen peroxide(H2O2)to establish cataract models in vitro.Meanwhile Rg1 were added to the culture medium and incubated for 24 hours.The anterior capsules were used in the following study.The expressions of apoptosis related genes Bcl-2 and Bax protein were studied by immunohisto chemical techniques.Results:Bcl-2 and Bax protein were expressed in LEC of normal rabbit lens.Expression of Bcl-2 protein was much stronger than that of Bax protein.The expression of Bcl 2 protein was down-regulated and that of Bax protein was up-regulated in H2O2 group when compared with the control group,so the ratio of Bcl-2/Bax was lower.Rg1 up-regulated the expression of Bcl-2,and down-regulated that of Bax protein;the ratio of Bcl-2/Bax was higher.There were significant differences in the range of ratio changes compared to that of the taurine group.Conclusion:Rg1 can up-regulate the expression of Bcl-2,and down-regulate that of Bax protein,to increase the ratio of Bcl-2/Bax;while the expression of Bcl-2 protein was down-regulated and that of Bax protein was up-regulated in the H2O2 group.The molecular mechanisms of both H2O2 inducing apoptosis of LEC and natural anti-oxidants restraining apoptosis of LEC may be related to the expressions of apoptosis-related gens Bcl-2 and Bax.更多还原