【摘要】 目的:研究铝过负荷致小鼠神经元退行性变与脑内氧化应激系统和抗氧化应激系统之间的关系。方法:采用氯化铝溶液(相当于400 mg/kg Al3+)灌胃小鼠,每天1次,1周6天,连续3月,建立铝过负荷所致小鼠神经元退行性变模型。以被动回避性学习记忆能力和空间识别能力的改变、大脑皮层和海马总SOD、SOD2活性和MDA含量变化、以及海马病理学改变为观察指标。结果:铝过负荷能明显导致小鼠进行性被动回避性学习记忆和空间识别能力障碍;出现海马神经元核固缩和神经元丢失;脑组织总SOD和SOD2活性及MDA含量呈平行变化,出现先升高后降低趋势,但各时点SOD和SOD2活性及MDA含量均明显高于对照组。结论:铝过负荷明显致小鼠神经元退行性变,学习记忆能力下降。其机制可能与铝负荷引起脑内氧化应激反应和抗氧化应激系统动员失恒有关。更多还原

【Abstract】 Objective: To study the roles of the oxidative stress system and the anti-oxidative stress system in neurodegenerative processes induced by aluminum overload in mice.Methods: The neurodegenerative model of mice was established via intragastric administration of AlCl3 solution(element aluminum 400 mg/kg)once a day,with 6 days of week for 3 months.The light-dark test and morris-water-maze were used to evaluate the learning and memory functions.The activities of SOD and SOD2 as well as the contents of MDA in both cortices and hippocampi were measured.Pathomorphological changes of hippocampi were observed.Results: Aluminum overload significantly decreased capacity of passive avoidance learning and memory and spatial recognition,caused the pyknosis and neuron loss of hippocampi,and parallelly increased SOD and SOD2 activities as well as MDA content in cortex and hippocampi,with a tendency of raising first and then gradually decreasing.Conclusions: Aluminum overload markedly causes neurodegeneration and damages learning and memory function in mice,the mechanism of which may be related to the imbalance between oxidative stress and anti-oxidative stress systems in brain induced by aluminum overload.更多还原