【摘要】 目的:利用5′-RACE技术进行人肝再生增强因子(hALR)相互作用肽cDNA5′末端快速扩增并进行序列分析。方法:按照5′-RACE试剂盒(Takara)操作流程进行hALR相互作用肽cDNA5′末端快速扩增,用BLAST技术对扩增序列进行生物信息学分析。结果:成功获取487bp的5′-RACE反应产物,其中5′端延伸了275bp,同源序列分析表明与人Citron激酶mRNA高度同源。结论:5′-RACE技术可以用于快速有效扩增已知部分cDNA序列的5′末端,Citron激酶可能是与hALR具有相互作用的蛋白。更多还原

【Abstract】 Objective: To amplify the 5′ cDNA end of human augmenter of liver regeneration(hALR) by 5′-RACE technique and to analyze the sequence.Methods: 5′ cDNA end of hALR was rapidly amplified by following the instructions of 5′-RACE kit(Takara).The acquired sequence was analyzed by bioinformatic tool.Results: 5′-RACE product with 487bp was successfully obtained,which included extending sequence with 275bp.Homology analysis by BLAST showed highly homologous with human citron kinase.Conclusions: 5′-RACE method can be used to amplify the 5′ end of partially known cDNA sequence.Citron kinase can interact with hALR.更多还原