【摘要】 目的:观察复方烫疡油(FTY)对兔耳腹侧瘢痕形成的影响,探索其作用机制,为临床应用提供依据。方法:用18只新西兰大白兔耳部腹侧制作创伤模型,于每侧兔耳腹侧作四个创面,每个创面1.2cm×1.0cm切除全层皮肤。获144个创面。将兔随机分为两组(A、B组)。A组全部右耳用FTY治疗;全部左耳用复方烫疡油赋形剂(灭菌的芝麻油)治疗;B组全部右耳用磺胺嘧啶银霜治疗;全部左耳用生理盐水治疗。于创伤后30,60,90天分别取创面组织作组织学观察,并用免疫组织化学法检测Ⅰ、Ⅲ型胶原、转化生长因子-β1(TGF-β1);TUNEL法定量检测组织细胞的凋亡指数。结果:FTY组创面愈合时间较其它组提前约1.5天。创面增殖块中组织中I、III胶原比值、TGF-β1、细胞核增殖抗原(PCNA)表达较其它组低,凋亡指数FTY组明显高于其它组。结论:复方烫疡油不仅能促进创面愈合,而且能减少创面瘢痕形成。更多还原

【Abstract】 Objective: To investigate the effects of Fu Fang Tang Yang You(复方烫疡油) on the formation of proliferation scars at the ventral side of the ear of rabbits and to explore its possible mechanism.Methods: 144 wounds at the ventral side of the ears of 18 New Zealand white rabbits were created,The area of each wound was 1.2cm×1.0cm.The wounded rabbits were randomly divided into two groups(groups A and B).All the wounds on the right ears of the rabbits in group A were treated with FTY;and the wounds on the left with vegetable oil.All the wounds on the right ears of group B were treated with Sulfadiazine silver cream;and the wounds on the left with normal saline.Tissues from the wounds were examined respectively.30d,60d,90d after injury.Each sample was immunohistochemically stained and examined TGF-β1,type of collagenⅠ?Ⅲ and proliferating cell nuclear antigen(PCNA) monoclonal antibody were examined.The apoptosis index was calculated with TUNEL technique.Results: In FTY group,the time of wound healing was reduced by about 1.5 days;The expression of TGF-β1?PCNA in the tissues of the wounds was lower than that of the control group.Meanwhile,the apoptotic index of fibroblasts was obviously higher than that of the control group.Conclusion: FTY can promote wound healing with less scar formation.更多还原