【摘要】 目的探讨高压氧(HBO)实验性治疗对大鼠急性坏死性胰腺炎(ANP)血内毒素、sCD 1 4及血浆灭活内毒素能力(EIC)的影响及其相关机制。方法将SD大鼠随机分为对照组、假手术组、ANP组和ANP+HBO(治疗)组。ANP模型建立3 0m in后,治疗组给予HBO治疗2 h。测定各组建模3,6,1 0 h后的血内毒素水平,sCD 1 4,EIC,TXB2和6-K-PGF1a水平。结果建模3 h和6 h后,治疗组的内毒素水平、sCD 1 4和TXB2水平明显低于ANP组(P<0.0 5),而EIC明显高于ANP组(P<0.05)。结论高压氧对大鼠ANP的治疗可能系通过改善胰腺微循环,增强EIC,降低血内毒素和sCD 1 4水平而实现。更多还原

【Abstract】 Objective To study the effect of hyperbaric oxygen（HBO） treatment on plasma endotoxin,sCD14 and plasma endotoxin inactivation capacity（EIC） in acute necrotizing pancreatitis（ANP） in rats and its possible mechanism.Methods SD rats were randomly divided into control group,sham operation group,ANP group and ANP+HBO group.Rat ANP models were made by retrograde injection of 3.5% sodium（taurocholate）（2.5mL/kg） into the pancreatic duct.Thirty minues after models had been made,ANP+HBO group was treated by hyperbaric oxygen for 2h.At 3h,6h,and 10h after establishment of rat models,the plasma endotoxin,sCD14,EIC,TXB₂ and 6-K-PGF_1a leves were determined in each group.Results At 3h and 6 h after rat models were established,the levels of endotoxin,sCD14,and TXB₂ in ANP+HBO group were significantly lower than those in ANP group（P< 0.05）;EIC level in ANP+HBO group was significantly higher than that in ANP group（P< 0.05）.Conclusions Through improvement of（microcirculation）,HBO could increase the level of EIC and decrease the level of endotoxin and sCD14 in ANP rats.更多还原