【摘要】 目的探讨炎性细胞因子IL-6、IL-8、IL-10及TNF-α在急性下肢深静脉血栓形成(DVT)疾病中的水平及意义。方法选择未经治疗的急性下肢DVT患者40例为DVT组,年龄、性别比无统计学差异的健康志愿者30例作为正常对照组。运用放射免疫分析检测血浆IL-6、IL-8及TNF-α水平,用ELISA法检测血浆IL-10水平。并对DVT组内细胞因子水平分别进行相关性分析,探讨各细胞因子之间的关系。结果相对于正常对照组,DVT组细胞因子水平均明显升高(P均<0.001)。DVT组内相关性分析,IL-6与IL-8及IL-10之间无相关性,IL-8与IL-10及TNF-α之间无相关性;IL-6与TNF-α之间呈正相关(r=0.383,P<0.05),IL-10与TNF-α之间呈正相关(r=0.390,P<0.05)。结论在DVT患者外周血中,均检测到血浆细胞因子的升高,炎性细胞因子可能在静脉血栓形成疾病中起重要的作用。更多还原

【Abstract】 To study the significance of the levels of plasma inflammatory cytokines (IL-6,IL-8,IL-10 and TNF-α) in patients with acute deep venous thrombosis (DVT) of lower extremity. Methods Forty untreated DVT cases were selected as the subjects in the DVT group, while thirty healthy subjects, whose ages and genders showed no significant difference with the DVT patients, were collected as the control group. The plasma levels of IL-6, IL-8 and TNF-α were detected by radioimmunoassay (RIA), and the plasma level of IL-10 was measured by enzyme-linked immunosorbent assay (ELISA). Correlation analysis was used to investigate the relationships between the levels of different inflammatory cytokines within DVT group. Results The levels of plasma cytokines in the DVT group were all significantly higher than those in control group (P<0.001). The results of the correlation analysis showed that there were positive correlations between IL-6 and TNF-α (r=0.383, P<0.05), IL-10 and TNF-α (r=0.390, P< 0.05), respectively, within the DVT group; whereas there were no correlations between IL-6 and IL-8, IL-6 and IL-10, IL-8 and IL-10, and IL-8 and TNF-α. Conclusion The levels of plasma cytokines increased significantly in patients of DVT. Inflammatory cytokines may play an important role in acute DVT by accelerating the pace of thrombosis, intensifying the inflammatory reaction around thrombus and aggravating the injured blood vessel.更多还原