【摘要】 【目的】赤眼蜂(Trichogrammaspp.)是全世界害虫生物防治中研究最多、应用最广的一类卵寄生性天敌,赤眼蜂蜂种的正确鉴别是取得良好防治效果的关键。【方法】通过对松毛虫赤眼蜂(Trichogrammadendrolimi)、玉米螟赤眼蜂(T.ostriniae)、螟黄赤眼蜂(T.chilonis)、短管赤眼蜂(T.pretiosum)、卷蛾赤眼蜂(T.cacoecia)5种赤眼蜂,及松毛虫赤眼蜂(T.dendrolimi)、玉米螟赤眼蜂(T.ostriniae)、螟黄赤眼蜂(T.chilonis)的不同地理种群的细胞色素氧化酶Ⅱ(mtDNACOⅡ)基因序列片段的测定,并调用GenBank中微小赤眼蜂T.minutum、T.plantneri的同源序列,对不同种赤眼蜂的mtDNACOⅡ序列进行了多重排比和聚类,探讨了mtDNACOⅡ用于赤眼蜂不同种系统进化分析及分子鉴定的可行性。【结果】序列分析结果表明,不同地理种群赤眼蜂的遗传距离均小于0.66%,不同种赤眼蜂间的遗传距离在2%～7.6%之间。【结论】COⅡ基因序列在赤眼蜂种内非常保守,而种间存在明显的遗传差异,可以用作赤眼蜂种的分子鉴定。更多还原

【Abstract】 【Objective】Trichogramma spp. is the most widely studied and used egg parasitoids for biological control of insect pests in the world. Correct Trichogramm species identification is the first step for a successful biological control program. 【Method】 The mitochondrial cytochrome oxidase Ⅱ(mtDNA COⅡ) of five Trichogramma species, i.e. Trichogramma dendrolimi, T. ostriniae, T. chilonis, T. pretiosum, T. cacoeciae and different populations of T. dendrolimi, T. ostriniae, T. chilonis were sequenced, and the homological sequences available in GenBank were retrieved for DNA alignment and clustering. Research focused on feasibility of the application of COⅡ sequences to the phylogenetic and molecular identification of different Trichogramma species and different geographical populations of the same Trichogramma species. 【Result】 Alignment of sequenced COⅡ showed genetic distances were less than 0.66% with Trichogramma speices and 2%-7.6% between Trichogramma speices. 【Conclusion】It can be concluded that COⅡ gene sequences were highly conserved within intraspecific taxa, but significant genetic difference existed between interspecific taxa, and COⅡ is suitable for molecular identification at the species level.更多还原