【摘要】 【目的】验证pinA和pinB对小麦籽粒质地的影响。【方法】将含有控制硬度的主效基因pinA和pinB的单子叶植物高效表达载体pUBPa和pUBPb,分别通过花粉管通道法将其转入小麦品种中优9507(pinB-D1b)中。【结果】获得了转基因植株,PCR检测和基因组Southern杂交证实了外源基因在受体小麦基因组中的整合,水洗淀粉表面蛋白的定量分析表明外源基因的导入影响了friabilin表达。与非转基因对照相比,小麦籽粒质地发生变化。【结论】结果表明,PINA和PINB共同作用形成friabilin,从而直接影响小麦籽粒质地的形成。更多还原

【Abstract】 【Objective】Grain hardness is an important factor in determining wheat end-use quality and one of the most important targets in wheat quality improvement. 【Method】In this study, two monocotyledonous expression vectors pUBPa and pUBPb harboring pinA and pinB genes, the major genes controlling grain hardness, were transferred into wheat variety Zhongyou 9507 (pinB-D1b) via pollen-tube pathway, respectively, and transgenic plants were obtained. 【Result】The integration of the foreign puroindolines genes was confirmed by both PCR and genomic Southern blot analysis. Quantitative analysis of the friabilin on the surface of water-washed starch granules showed that the integration of foreign puroindolines genes indeed affected the expression level of puroindolines and grain texture. 【Conclusion】In conclusion, PINA and PINB (Puroindoline) interact to form friabilin and affect wheat grain texture.更多还原