【摘要】 目的本文研究了信号分子一氧化氮(NO)和过氧化氢(H2O2)对水稻细胞过敏反应(HR)的诱导作用。方法用溴酚兰染色法定量检测这两种信号分子单独或协同诱导作用下水稻细胞的HR。结果该两种信号分子可以独立或协同诱导水稻细胞HR的产生,H2O2对NO诱导的HR具有调节作用,但NO与H2O2浓度平衡与否对HR诱导并无影响。结论H2O2和NO均是诱导水稻细胞HR的信号分子,诱导过程中并不存在“NO/H2O2浓度平衡作用机制”。更多还原

【Abstract】 【Objective】The hypersensitive response(HR)in rice suspension cultured cells induced by exogenous addition of the signal molecules,nitric oxide(NO)and hydrogen peroxide(H2O2)was studied.【Method】The HR of rice cells induced by NO and H2O2 dependently and/or in synergy,was quantitatively determined by the bromphenol blue staining method【.Result】Results showed that HR of rice cells could be induced by NO and H2O2 dependently and/or in synergy and that NO-induced HR could be regulated by H2O2.The balance interaction in concentrations between NO and H2O2 had no influence on the induction of HR【.Conclusion】NO and H2O2 were the signal molecules for the induction of the rice cells HR and the balance model for NO and H2O2 interaction was not proved in the induction of the HR of rice cells.更多还原