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甘蔗宿根矮化病菌PCR检测及目的片段核苷酸序列分析
Detection of Sugarcane Ratoon Stunting Disease Pathogen with Polymerase Chain Reaction(PCR) and Nucleotide Sequence Analysis
【摘要】 根据甘蔗宿根矮化病(RatoonStuntingDisease,RSD)病原细菌(Leifsoniaxylisubsp.xyli,Lxx)16S-23SrDNA基因间隔区核苷酸序列设计的一对特异性引物,建立了甘蔗宿根矮化病PCR检测方法;同时对PCR扩增的目的片段核苷酸序列进行测定与分析。结果表明广东湛江蔗区RSD病菌16S-23SrDNA基因间隔区核苷酸序列与巴西、澳大利亚及美国路易斯安娜州RSD株系基因组相应区段核苷酸同一率接近100%,病菌高度的同源。而与近缘的木质部赖氏杆菌狗牙根变种(Leifsoniaxylisubsp.Cynodontis,Lxc)和形态上相近的马铃薯环腐病菌(Clavibactermichiganensissubsp.Michiganensis)基因组相应区段核苷酸同一率较低,存在明显的分化。
【Abstract】 PCR technique was established for detection of Leifsonia xyli subsp. xyli(Lxx.), the causal agent of sugarcane ratoon stunting disease(RSD), with specific primers based on the intergenic transcribed spacer(ITS) region of 16S-23S ribosomal DNA of Lxx.By sequence comparison of intergenic transcribed spacer(ITS) region of 16S-23S rDNA, it was revealed that RSD pathogen was highly conversat. Lxx guangdong isolate shares almost 100% nucleotide identity with Brazil,Australia and USA isolates. However,Lxx ITS sequences share only low identity with related close(Leifsonia xyli subsp. Cynodontis,Lxc) and morphologic similar bacteria(Clavibacter michiganensis subsp.michiganensis).
【Key words】 Sugarcane； Ratoon stunting disease； PCR technique； Nucleotide sequence analysis；
- 【文献出处】 中国农学通报 ,Chinese Agricultural Science Bulletin , 编辑部邮箱 ,2006年12期
- 【分类号】S435.661
- 【被引频次】36
- 【下载频次】209