【摘要】 山桐子是具有较高观赏树种和利用的树种。通过正交试验等方法对山桐子茎段组织培养外植体灭菌、玻璃苗预防、增殖培养、根诱导等关键技术进行研究。结果表明:在南京山桐子最佳取材时间为4月下旬,在引进日本种源优良母株上选取外植体,经过0.1%HgCl2消毒15s,灭菌成活率为76.7%;添加3.5%的白砂糖和增加培养容器的透气性,能很好地预防玻璃苗的发生;改良MS附加TDZ、BA、NAA等植物生长调节剂可有效促进试管芽苗增殖和生长,其中TDZ0.05mg/L+BA1.5mg/L+NAA0.05mg/L组合最适宜增殖与生长,增殖倍数达5.3,生长高度为2.7cm;适宜的生根培养基为1/2MS+IBA0.5mg/L+NAA0.3mg/L+LH100mg/L+S2%,生根率可达87%;经生根的试管苗移栽于蛭石(V):珍珠岩(V):泥炭(V)=6:3:1的混合基质,控制温度20￣30℃,相对湿度85%￣90%,保湿15￣20d,其间适当遮荫,30d后成活率达85%以上。更多还原

【Abstract】 Idesia polycarpa Maxim. is an plant with highly appreciative and useful value.This experiment concerning Idesia polycarpa Maxim.segment culture in vitro was committed on the explant sterilization, shoot vitrification control, shoot multiplication and root induction. The results showed that:(1)as for the one-year-old mother tree acclimated at Nanjing, Jiangsu Province, formerly introduced from Japan, the latter of April was optimal time in explant collection. After 0.1% HgCl2 sterilization for 15s,the explants suitful for the forthcoming culture accounted up to 76.7%;(2)addition of 3.5% sugar and air-permeation improvement to culture containers could effectively controll shoot vitrification;(3)the modified MS media supplemented with TDZ, BA and NAA could ameliorate the culture multiplication and growth,among which the combination of 0.05mg/L TDZ + 1.5 mg/L BA + 0.05 mg/NAAL was optimal, with the multiplication time up to 5.3 and growth height up to 2.7cm;(4) the rooting medium, i.e. 1/2MS+ 0.5 mg/L IBA + 0.3 mg/L NAA + 100 mg/L LH + 2% S was optimised ,with rooting percentage up to 87%;(5) the root-developed plantlets transplanted in the substrate mixed with vermiculite, perlite and peat (volumn ratio of 6:3:1) at 20~30℃, relative humidity of 85%~90% for15~20d, favorably shaded, could get over 85% survival after 30d.更多还原