【摘要】 以六个品种大豆体细胞胚为受体,以Bt基因为目的基因,应用根癌农杆菌介导法对大豆进行了遗传转化,经卡那霉素选择、诱导体细胞胚萌发及壮苗培养,获得了完整的抗性再生苗,经PCR、PCR-Southern和点杂交分析鉴定,初步证明外源基因Bt已整合到大豆的基因组中。更多还原

【Abstract】 Somatic embryos of 6 different soybean were transformed by Agrobacterium tumefaciens , The resistant plants to kanamycin were obtained. 5 positive plants were obtained among them by PCR , These positive plantlets were further analyzed by dot blotting and PCR-Southern blotting. These results demonstrated that the target gene had been transformed into the soybean genome.更多还原