【摘要】 以C18为分离柱,以100mmol/L磷酸钠缓冲溶液(pH2.1)/0.8mol/L辛基磺酸和乙腈=9:1(v/v)混合液为流动相,检测波长为270nm的液相色谱检测VB1含量,在0.005μg￣2.5μg范围内进样与主峰面积线性关系良好(r=0.9997),最小检测量0.0050μg,检出限0.0002μg。更多还原

【Abstract】 The content of vitamin B1 (VB1) was detected by high performance liquid chromatography (HPLC) at 270 nm, in which C18 Chromatography column was used. The mobile phase was a mixture containing acetonitrile and the solution of 100 mmol/L Na3PO4 (pH 2.1) and 0.8 mol/L octylsulfoacid, with the mix ratio of 1:9(v/v). The content of VB1 and the area of main peak presented good linear relationship (r=0.9997) when the content of VB1 ranged between 0.005 μg and 2.5 μg. The minimum injection dose was 0.0050 μg, and the detection limit was 0.0002 μg.更多还原