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产纤溶酶的米曲霉筛选及测定方法的研究
Screening of Aspergillus oryzae strains producing fibrinolytic enzyme and determination method of fibrinolytic enzyme
【摘要】 利用平板透明圈法作为初筛方法,从不同的酿造曲样中筛选出1株产纤溶酶酶活较高的米曲霉菌株SA-6,其产纤溶酶酶活为25U/mL。通过比较发现,可以用自制纤维蛋白代替纤维蛋白原和尿激酶测定发酵液的纤溶酶酶活,在大通量的菌株筛选过程中可以节省大量费用。
【Abstract】 A Aspergillus oryzae strain,SA-6,was isolated from different brewing koji samples using the clarity circle method on plate.The fibrinolytic enzymatic activity of SA-6 was 25 U/ml.It was showed through comparison that fibrinogen and thrombin couldbe replaced by fibrin to determine the activity of fibrinolytic enzyme,which could save much money during the screening of a large quantity of strains.
【关键词】 溶酶;
米曲霉;
筛选;
测定方法;
【Key words】 fibrinolytic enzyme; Aspergillus oryzae; screening; determination method;
【Key words】 fibrinolytic enzyme; Aspergillus oryzae; screening; determination method;
- 【文献出处】 中国酿造 ,China Brewing , 编辑部邮箱 ,2006年01期
- 【分类号】TS201
- 【被引频次】10
- 【下载频次】209