【摘要】 目的:通过观察乙型肝炎患者血清中sICAM-1和sVCAM-1的水平及其与ALT、AST相关性,探讨Ⅰ型超敏反应与乙型肝炎发病机制的关系。方法:应用酶联免疫吸附实验,检测45例乙型肝炎患者及15例对照组血清sICAM-1和sV-CAM-1水平;应用全自动生化分析仪检测患者血清ALT和AST水平,并观察与sICAM-1和sVCAM-1的相关性。结果:①7例急性肝炎患者血清中sICAM-1和sVCAM-1含量明显高于正常对照组;②38例慢性肝炎患者血清中sICAM-1和sVCAM-1含量也明显高于正常对照组;③13例中度慢性肝炎患者的sICAM-1和sVCAM-1含量显著高于16例轻度慢性肝炎患者;④9例重度慢性肝炎患者的sICAM-1和sVCAM-1含量也明显高于轻度组和中度组;⑤sICAM-1水平与血清内ALT、AST含量呈显著正相关;⑥sVCAM-1水平与血清ALT、AST含量也呈显著正相关。总之,在以上几组中,急性乙型肝炎组的sICAM-1和sVCAM-1的升高最显著,并依次为慢性肝炎重度组、中度组和轻度组。结论:①急、慢性乙型肝炎患者血清中sICAM-1和sVCAM-1水平可反映肝损害程度;②检测急、慢性乙型肝炎患者血清sICAM-1和sVCAM-1水平,对判断患者的病情和预后有一定的参考价值;③作为Ⅰ型超敏反应性炎症的重要指标,sICAM-1和sVCAM-1可能参与了乙型肝炎的发病和肝细胞的免疫损伤过程。更多还原

【Abstract】 Objective:To further explore the relationship between Type Ⅰ hypersensitivity reaction and the mechanism of the viral hepatitis B through the research of soluble intercellular adhesion molecular-1(sICAM-1) and soluble vascular cell adhesion molecular-1(sVCAM-1) levels in serum in patients with viral hepatitis B related to Type Ⅰ hypersensitivity reaction and the damage of liver cells, and therefore, provide new theory for the perfection of the immunological mechanism of hepatitis B, especially acute hepatitis B.Methods:Serum sICAM-1 and sVCAM-1 levels were measured in 45 patients with viral hepatitis B and 15 normal cases using double antibody sandwich ELISA method. ALT and AST levels were studied using omni automatic biochemistry analyzer and its correlation with sICAM-1 and sVCAM-1 were observed.Results:The sICAM-1 and sVCAM-1 levels of 7 acute hepatitis B were significantly higher than that of the normal cases. ②The sICAM-1 and sVCAM-1 levels of 38 chronic hepatitis B were also significantly higher than that of the normal cases. ③The sICAM-1 and sVCAM-1 levels of 13 cases moderate chronic hepatitis B were significantly higher than that of 16 cases mild chronic hepatitis B. ④The sICAM-1 and sVCAM-1 levels of 9 serious chronic hepatitis B were significantly higher than that of the group of mild chronic hepatitis B. ⑤Levels of sICAM-1 were significantly positively correlated with serum ALT and AST. ⑥sVACM-1 levels were significantly positively correlated with serum ALT and AST. In conclusion, sICAM-1 and sVCAM-1 levels in patients with acute hepatitis B were most remarkably increased, and then serious chronic hepatitis B, moderate chronic hepatitis B, light chronic hepatitis B in sequence.Conclusion:Serum sICAM-1 and sVCAM-1 levels in patients with viral hepatitis B may reflect the damage of liver. ②Examination of serum sICAM-1 and sVCAM-1 levels in patients with hepatitis B can be used to judge the patient’s condition and to diagnose. ③As an important indication of the inflammatory in Type Ⅰ hypersensitivity reaction, sICAM-1 and sVCAM-1 may interfere the appearence of hepatitis B and the procedure of immunological damage of liver cells.更多还原