【摘要】 目的:探讨人类白细胞抗原(HLA)配型、交叉反应组(CREGs)误配率与尸体肾移植术后早期急性排斥反应的关系。方法:应用泰萨奇单克隆抗体干板进行供受者HLA-Ⅰ类抗原分型;微量序列特异性引物法进行HLA-Ⅱ类基因分型;泰萨奇混合抗原板检测群体反应性抗体(PRA)。结果:PRA阴性131例肾移植患者HLA配型,误配率为6MM、5MM、4MM、3MM、2MM、1MM、0MM的移植例数分别为0、4、26、49、33、15、4,术后早期急性排斥反应发生率分别为0、25%、23.1%、14.3%、12.1%、6.7%、0。CREGs误配率为6MM、5MM时无移植病例。CREGs误配率为4MM、3MM、2MM、1MM、0MM时,排斥反应发生率分别为28.6%、22.9%、9.5%、6.9%、5.5%。结论:HLA配型、交叉组配型可显著提高供受者的HLA相配率,对选择最佳的供者,降低早期急性排斥反应的发生,提高肾移植效果具有重要的意义。更多还原

【Abstract】 Objective:To study the relationship between human leukocyte antigen(HLA) typing, HLA crossreaction antigen group(CREGs) mismatching and early rejection after cadaver donor kidney transplantation.Methods:Donor and recipient HLA class Ⅰ typing was performed with Paul Terasaki monoclonal antibody tray and HLA class Ⅱ gene typing with Micro-sequence-specific-primers(Micro-SSP). PRA was detected by using Paul Terasaki mixture antigen tray.Results:The cases of 6, 5, 4, 3, 2, 1, 0 HLA mismatching(MM) in 131 recipients with negative PRA were respectively 0, 4, 26, 49, 33, 15, 4. The incidence of early rejection were 0,25%,23.1%,14.3%,12.1%,6.7%,0. No recipient with 5 and 6 CREGs MM. The case of 4,3,2,1,0 CREGs MM, the early rejection were respectively 28.6%,22.9%,9.5%,6.9%,5.5%.Conclusion:HLA typing and HLA crossreactive antigen group(CREGs) matching play an important role in selecting suitable donor, reducing early rejection, improving the effect of kidney transplantation.更多还原