【摘要】 目的:研究替换CⅡ263-272序列中的268~270位氨基酸的变构肽sub268-270对胶原性关节炎(CIA)的抑制作用。方法:使用牛CⅡ在Lewis大鼠诱发CIA。以CⅡ变构肽sub268-27090μg静脉注射,每周1次治疗CIA;设置无关肽对照和空白对照组。从关节炎症积分、放射学积分和病理学积分来评价变构肽疗效。结果:变构肽、无关肽及空白对照组关节炎症积分分别为5.60±1.24、11.20±1.21和11.80±1.22,变构肽可明显抑制CIA关节炎症(P<0.01)。变构肽组的放射学积分(1.32±0.49)明显低于无关肽组(2.63±0.51)和空白对照组(2.69±0.53)(P<0.01)。此外,变构肽组的病理学积分(1.37±0.53)也显著低于无关肽组(2.94±0.63)和空白对照组(3.06±0.65)(P<0.01)。结论:CⅡ变构肽sub268-270可以明显抑制胶原性关节炎的关节炎症、病理损伤及骨破坏程度。更多还原

【Abstract】 Objective:To study the inhibition of collagen-induced arthritis by an altered CⅡ263-272 peptide(sub268-270) with three consecutive substitutions of TCR-contacting residues.Methods:Arthritis was induced by bovine collagen type Ⅱ. The altered peptide sub268-270 was given intravenously with a dose of 90 μg once a week. Peptide control and blank control were treated with similar approaches. The therapeutic effect of the altered peptide was evaluated by arthritis score index, radiological score and pathological score.Results:The arthritis score of rats treated with altered peptide, control peptide and blank control were 5.60±1.24, 11.20±1.21 and 11.80±1.22 respectively(P<0.01), implying that the altered CⅡ peptide could decrease arthritis score of CIA. The radiological score of rats treated with altered peptide, control peptide and blank control were 1.32±0.49, 2.63±0.51 and 2.69±0.53(P<0.01), showing that the altered CⅡ peptide could decrease CIA radiological lesions. The pathological score of rats treated with altered peptide, control peptide and blank control were 1.37±0.53, 2.94±0.63 and 3.06±0.65(P<0.01), suggesting that the altered CⅡ peptide could retard CIA pathological injury.Conclusion:Altered CⅡ peptide sub268-270 could effectively ameliorate arthritis in CIA.更多还原