【摘要】 目的:探讨趋化因子IP-10和细胞因子IFN-γ是否参与人缺血脑损伤过程。方法:将21例脑梗死死亡病例按发病持续时间分为<7天、7~14天和15~21天3组,以非缺血侧半球做对照,用HE染色法观察炎性细胞浸润情况;通过免疫组织化学方法检测缺血半球脑组织与非缺血半球脑组织中趋化因子IP-10和细胞因子IFN-γ的表达。结果:在<7天组和7~14天组中,缺血脑组织可见大量炎性细胞浸润。在<7天组、7~14天组和15~21天组中,趋化因子IP-10在缺血半球脑组织中的表达高于非缺血半球(分别是1·74倍增高,P<0·01;1·41倍增高,P<0·05和1·52倍增高,P<0·01)。在对细胞因子IFN-γ的检测中发现<7天和7~14天组中,IFN-γ在缺血半球脑组织中的表达高于非缺血半球(分别是1·65倍增高,P<0·05和1·32倍增高,P<0·05);在15~21天组中,IFN-γ在缺血半球与非缺血半球中的表达没有显著差异(P>0·05)。结论:在人缺血脑组织中观察到IP-10和IFN-γ的表达,提示IP-10和IFN-γ参与了炎症反应对脑组织的损伤过程。同时也提示IP-10可能参与后期对损伤脑组织的修复。更多还原

【Abstract】 Objective:To investigate whether interferon-gamma inducible protein-10(IP-10) and interferon-gamma(IFN-γ) participated in the human cerebral ischemia injury.Methods:Twenty-one cerebral ischemia specimens, collected from patients died of cerebral infarction, were divided into three groups: less than 7 days, 7-14 days and 15-21 days according to the lasting time of cerebral infarction. The infiltrating of inflammatory cells were observed using HE stain as non-ischemic hemisphere was for controls. Expression of IP-10 and IFN-γ in sections both postmortem ischemic hemisphere and non-ischemic hemisphere were detected using immunohistochemistry.Results:In the groups of less than 7 days and 7-14 days large quantity of inflammatory cells were infiltrated in ischemia tissue. Expression of IP-10 in three groups was elevated in the ischemic hemisphere compared with non-ischemic hemisphere(1.74-folds, 1.41-folds and 1.52-folds increases respectively, P<0.01, P<0.05, P<0.01). In the detection for IFN-γ, the expression of IFN-γ was elevated in the ischemic hemisphere compared with non-ischemic hemisphere in less than 7 days and 7-14 days (1.65-folds and 1.32-folds increases respectively, P<0.05). However, in 15-21 days, the expression of IFN-γ was not significant deviation both in the ischemic and non-ischemic hemisphere(P>0.05).Conclusion:These results showed IP-10 and IFN-γ are expressed in human cerebral ischemia. It was suggested that IP-10 and IFN-γ involve in cerebral ischemia injury. Moreover, it was also suggested that IP-10 participate in the repair for the later stage of cerebral ischemia injury.更多还原