【摘要】 目的应用聚合酶链反应(PCR)技术分析湖北省不同地区媒介按蚊的种型。方法采用传统的形态学方法和新建立的基因鉴别技术对现场捕获的按蚊分别进行形态特征鉴别和基因鉴别及比较。结果现场捕获181只按蚊,形态学确认176只为中华按蚊,而PCR鉴定172只为中华按蚊,另4只为八代按蚊;经形态学特征鉴别的5只嗜人按蚊中,PCR鉴别有4只为嗜人按蚊,另1只为八代按蚊。结论采用PCR基因鉴别技术能准确鉴别赫坎按蚊种团内中华按蚊、嗜人按蚊、八代按蚊等近缘种按蚊,较传统的按蚊形态学鉴别方法准确,适用于复合媒介地区的疟疾媒介调查和监测。更多还原

【Abstract】 Objective To identify species of anopheles vectors captured in varied areas of Hubei province with polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） techniques.Methods Anopheline mosquitoes（Anopheles hyrcanus complex） captured at the fields of Hubei province were identified with traditional morphological classification and newly-established genetic methods（PCR-RFLP） based on the sequences of their ribosomal DNA in the ITS₂ region.DNA sequence in Anopheles anthropophagus,Anopheles sinensis and Anopheles yatsushiroensisi were analyzed and compared. Results（A total） of 181 anopheline mosquitoes were captured and identified,including 176 identified as Anopheles sinensis and five as Anopheles anthropophagus according to their morphological features,and 172 identified as Anopheles sinensis,four as Anopheles anthropophagus and one as Anopheles yatsushiroensisi by PCR-RFLP.Conclusion Close-breeding anopheles,such as Anopheles sinensis,Anopheles yatsushiroensisi and Anopheles anthropophagus in Anopheles hyrcanus complex could be accurately identified by PCR-RFLP,which was suitable for the investigation and surveillance for malaria-vector mosquitoes in the areas with anopheles complex.更多还原