【摘要】 采用随机区组和正交试验设计,对蓝靛果芽体组织培养技术进行了系统研究.结果表明:外植体以冬眠枝条经水培后萌发的嫩枝茎段为最好;最佳消毒方法为0.1%HgCl2消毒6 min;最佳培养基为MS+6-BA(1.0 mg/L)+IBA(0.2 mg/L).更多还原

【Abstract】 Using the experiment design of random and orthogonal tests,technique of tissue culture of Lonicera edulis’ shoot were studied.The results are shown: The best explant is the delicate shoot after Winter; The best disinfect method is（0.1%） HgCl₂,disinfect time is 6 minutes;The best culture medium is MS+6-BA（（1.0 mg/L））+IBA（（0.2 mg/L））.更多还原