【摘要】 以克伦特罗(CL)为竞争半抗原,人工合成的克伦特罗-卵清白蛋白(CL-OVA)为包被抗原,两者与一定量的SD大鼠抗CL血清反应,建立快速定量测定CL含量的间接竞争酶联免疫吸附检测(ciELISA)方法。结果表明,理想的包被抗原质量浓度为3μg/mL,抗CL血清稀释倍数为1∶1.6×105。绘制出标准曲线,线性检测范围为4.88~5 000ng/mL,线性回归方程为y=-0.295 2x+1.160 6,r2=0.990 2,加标平均回收率为95.45%。该方法敏感性高,特异性强,操作简便,对于研制瘦肉精残留检测试剂盒有重要意义。更多还原

【Abstract】 Clenbuterol（CL） acted as competitive hapten,the conjugate OVA-CL of ovalbumin and clenbuterol acted as coating antigen,competitive indirect ELISA（ciELISA） for clenbuterol have been developed based on Sprague-Dawley（SD） rat antiserum against CL.The obtained optimum conditions of the assay follow: 3μg/mL of the plate-coating antigen CL-OVA in carbonate sodium buffer,1∶1.6×10⁵ of antiserum titer.The detection range of this assay method is broadly various from 4.88ng/mL to 5 000ng/mL,and an average recovery of 90.67% is obtained.This method shows high sensitivity,strong specificity.更多还原