【摘要】 分别采用自由基对荧光试剂的氧化消光法和生物化学发光法对黑米、黑大豆、黑玉米3种黑色粮油作物种皮提取物进行体外总抗氧化能力和清除自由基作用的比较研究。结果表明:总抗氧化能力强弱顺序为黑大豆>黑米>黑玉米,其ORAC值分别为5．29 U／mL,3．13 U／mL和2．25 U／mL;在实验浓度0-0．5 mg／mL范围内,3种黑色粮油作物的种皮提取物对O2-·、·OH和H2O2均有不同程度的清除作用,并呈现量效关系;清除O2-·作用顺序为黑大豆>黑米>黑玉米,其IC50分别为0．455mg／mL,0．465mg／mL,0．488mg／mL; 清除·OH作用顺序为黑大豆>黑玉米>黑米,其IC50分别为0．059mg／mL,0．142mg／mL,0．199mg／mL;清除 H2O2．作用顺序则为黑大豆>黑米>黑玉米其IC50分别为0．079 mg／mL,0．123mg／mL,0．483mg／mL。更多还原

【Abstract】 The antioxidation capacity in vitro and scavenging free radical capacity of seed pericarp extracts of black rice, black soybean and black corn was compared by means of oxidative extinction of free radical acting on fluorescence regent and biological chemiluminescence. The following results are shown in the paper. The total antioxidation capacity order is black soybean > black rice > black corn,with oxygen radical absorption capacity(ORAC) value of 5.29 U/mL,3. 13 U/mL, and 2.25 U/mL respectively. Within the experimental concentration range of 0 ~ 0.5mg/mL,the three black crop seeds can scavenge O2-· , ·OH, and H2O2 at varying degree,showing dose effect relation. The O2 -· scavenging actions order is black soybean > black rice > black corn, with IC50 value of 0.455mg/ mL, 0.465mg/mL, and 0.488mg/mL respectively. The ·OH scavenging action order is black soybean > black corn > black rice,with IC50value of 0. 059mg/mL,0. 142mg/mL, and 0.199mg/mL respectively. The H2O2 scavenging action order is black soybean > brown rice > black corn,with IC50value of 0.079mg/mL,0. 123mg/mL, and 0.483 mg/mL respectively.更多还原