【摘要】 目的研究以bcl-2基因为靶标的有效siRNA对HL-60细胞高三尖杉酯碱(HT)药物敏感性的影响。方法通过脂质体将siRNA转入HL-60细胞株并与HT联合培养,于24,48,72h,用MTT法检测其对细胞生长的抑制,用流式细胞仪检测HL-60细胞bcl-2蛋白的表达率,细胞内活性氧(ROS)水平变化及细胞线粒体膜电位的变化。结果siRNA明显提高HT对HL-60细胞的生长抑制效应,抑制细胞bcl-2蛋白的表达,提高细胞内ROS水平,降低线粒体膜电位(P<0.05)。结论以bcl-2基因为靶标的siRNA能提高白血病细胞HL-60对HT的敏感性。更多还原

【Abstract】 Objective To study the effect of siRNA targeting bcl-2 gene on the drug-sensitivity of HL-60 cell to homoharringtonin(HT). Methods siRNA, selected by previous experiments and packaged with lipofectamine 2000, was transferred into HL-60 cells. Six hours later, the cells were cultured with homoharringtonin. The cell growth of HL-60 cells was detected by MTT methods at 24, 48, 72 hours respectively. The level of bcl-2 protein and ROS (reactive oxygen species, ROS) as well as membrane potential of mitochondrion was determined by flowcytometry. Results The siRNA could significantly increase the inhibitive effect of homoharringtonin on growth of HL-60 cells. The combination of siRNA with homoharringtonin resulted in the falling of bcl-2 protein level and rising of ROS level as well as descending of membrane potential of mitochondrion of HL-60 significantly (P < 0.05). Conclusion The effective siRNA targeting bcl-2 can increased the drug-sensitivity of HL-60 leukemic cells to homoharringtonin by inhibiting the expression of bcl-2 protein.更多还原