【摘要】 目的观察益智口服液对阿尔茨海默病模型大鼠不同脑区凋亡相关基因及蛋白质的影响。方法40只成年雄性清洁级W istar大鼠,体重250~300 g,随机取10只为正常组,另30只利用凝聚态Aβ1-40对W istar大鼠海马CA1区进行定位损伤建立AD模型。模型鼠随机分3组(益智口服液组、褪黑素组、生理盐水对照组,每组10只),分别服用益智口服液、褪黑素和生理盐水。40 d后处死大鼠,应用分子生物学、免疫组化等方法对AD鼠脑内细胞色素C蛋白质表达、白介素-1β转化酶mRNA、B细胞淋巴瘤mRNA等方面进行研究。结果益智口服液可以降低细胞色素C蛋白质表达、减少白介素-1β转化酶mRNA含量、升高B细胞淋巴瘤mRNA含量。结论益智口服液可在不同环节对细胞凋亡产生影响,对抗凋亡的发生,对Aβ的神经毒性损伤具有保护性作用,改善AD模型鼠痴呆状况。更多还原

【Abstract】 Objective To observe the effect of Chinese medicine（Yizhikoufuye,YZ） on related genes and proteins of cell apoptosis in the different cerebral regions of experimental Alzheimer rats.Methods Forty male Wistar rats,weighed 250～300 g,were randomly and averagely divided into normal group,YZ group, melatonin group and saline group（these 30 rats were damaged by Aβ₁～40 in CA1 to build Alzheimer’s diseases model）.The rats were killed 40 days later to observe cytochrome C protein expression,IL-1β converting enzyme（ICE） mRNA,Bcl2 mRNA in brain of AD rats with molecular biology and immunohistochemical assay.Results YZ decreased cytochrome C expression and ICE mRNA content and increased Bcl-2 mRNA content.Conclusions YZ could influence the different links of cellular apoptosis,antagonize apoptosis,have protective effect on neurological toxicity induced by Aβ to improve the syndrome of AD model rats.更多还原