【摘要】 目的研究经肝动脉插管灌注碘油抗癌药乳液化疗栓塞和单纯灌注化疗后的早期兔肝VX2肿瘤发生凋亡。方法27只新西兰大白兔瘤组织块种植肝VX2肿瘤,A组(n=9)经肝动脉灌注碘油抗癌药乳液,B组(n=9)肝动脉灌注化疗,C组(n=9)肝动脉灌注生理盐水,每组分别在处理后第24h、72h、120h处死实验兔各3只,采用FITC-AnnexinV/PI流式细胞术分析介入处理后早期的肿瘤细胞凋亡。结果肿瘤边缘部的凋亡早期细胞百分率(PapE)与凋亡晚期细胞百分率(PapL)及峰值时间点分别为:A组(9.48±4.58)%、(33.46±8.81)%、24h、72h,B组(4.46±1.47)%、(11.65±3.51)%、24h、24h,C组(2.50±1.39)%、(5.99±1.22)%。肿瘤中心部的PapE、PapL与峰值时间点分别为:A组(16.48±6.32)%、(43.56±18.51)%、24h、72h,B组(7.50±3.10)%、(18.53±14.04)%、24h、24h,C组(5.61±1.92)%、(14.63±12.23)%。结论Lp-THACE诱导肝癌肿瘤细胞早期大量凋亡是其有效的主要作用机制,其诱导凋亡作用明显大于THAI。更多还原

【Abstract】 Objective To research the tumor cell apoptosis with flow cytometry for rabbit VX2 liver implanted tumor in earlier period after Lp-THACE and THAI. Methods 27 New Zealand white rabbits were implanted VX2 tumor at left middle lobe of the liver,were divided into three groups which treated by transhepatic artery catheterrizeation:Group A(n=9),Lp-THACE; Group B(n=9),THAI; Group C(n=9) as control.At 24h,72h,and 120h, three rabbits in each group were executed respectively. FITC-AnnexinV/PI were used for measuring apoptosis by flow cytometry. Results The percentage of apoptosis early and late cell (PapE, PapL), peak value time at tumor marginal area respectively were: ( 9.48 ± 4.58 )%、( 33.46 ± 8.81 )%、 24h、72h in group A, ( 4.46 ± 1.47 )%、( 11.65 ± 3.51 )%、24h、24h in group B, ( 2.50 ± 1.39 )%、( 5.99 ± 1.22 )% in group C. PapE,PapL,peak value time at tumor centre area resspectively were: ( 16.48 ± 6.32 )%、( 43.56 ± 18.51 )%、 24h、72h in group A, ( 7.50 ± 3.10 )%、( 18.53 ± 14.04 )%、24h、24h in group B, ( 5.61 ± 1.92 )%、( 14.63 ± 12.23 )% in group C. Conclusion It was a primary mechanism for Lp-THACE that induce hepatic cancer to take place considerable tumor cell apoptosis in earlier period, and the strength of Lp-THACE induce apoptosis was obvious larger than THAI.更多还原