【摘要】 目的探讨16、18型人乳头瘤病毒(HPV16/18)在宫颈腺癌发生发展中的作用,分析HPV16/18感染与环氧化酶-2(COX-2)蛋白表达的关系。方法采用组织微阵列技术结合原位杂交和免疫组化(SP法)检测24例慢性宫颈炎和86例宫颈腺癌HPV16/18DNA和COX-2蛋白表达情况。结果HPV16/18DNA和COX-2蛋白在宫颈腺癌中的阳性表达率分别为65.1%与86.0%,均显著高于慢性宫颈炎组织8.3%与33.3%(P<0.01)。HPV16/18感染与宫颈腺癌的病理分级和组织学类型无关,但与COX-2表达呈正相关(P<0.05)。COX-2表达与宫颈腺癌的病理分级有关,G3组COX-2蛋白阳性表达率明显高于G1组(P<0.05)。COX-2表达与宫颈腺癌组织学类型无明显相关性(P>0.05)。结论宫颈腺癌的发生与HPV16/18感染及COX-2蛋白表达异常相关,宫颈腺癌中COX-2高表达与HPV16/18感染有一定相关性,二者可能协同作用导致宫颈腺癌恶性发展。更多还原

【Abstract】 Objective To study the role of 16,18 human papilloma virus (HPV16/18) infection in the carcinogenesis of cervical adenocarcinoma,and to analyze the relationship between HPV16/18 infection and cyclooxygenase-2(COX-2) protein expression.Methods The expressions of HPV16/18DNA and COX-2 protein were determined by tissue microarray combined with in situ hybridization and immunohistochemistry in 86 cases of cervical adenocarcinoma and 24 cases of cervical chronic inflammation.Results The positive rates of HPV16/18DNA and COX-2 protein in cervical adenocarcinoma were 65.1% and 86.0% respectively,which significantly higher than that in cervical chronic inflammation 8.3% and 33.3% (P< 0.01 ).There is no relationship between HPV16/18 infection and the pathological grades or histological subtypes,otherwise,there is positive correlation between HPV16/18 infection and COX-2 expression (P< 0.05 ).The expression of COX-2 is related to the pathological grades,the positive rate of COX-2 is significantly higher in G_ 3 than in G_ 1 (P< 0.05 ).There is no relationship between COX-2 expression and the histological subtypes(P> 0.05 ).Conclusion Carcinogenesis of cervical adenocarcinoma may be related to the infection of HPV16/18 and the abnormal expression of COX-2 protein.Their synergistic reaction might lead to the development of cervical adenocarcinoma.更多还原