【摘要】 目的研究乳腺癌组织和癌旁组织O6甲基鸟嘌呤DNA甲基转移酶(MGMT)和谷胱苷肽S转移酶π(GSTπ)蛋白表达的临床意义。方法应用流式细胞术(FCM)分析MGMT和GSTπ蛋白表达。结果乳腺癌组织MGMT和GSTπ表达显著高于癌旁组织(P<0.05)。2种耐药相关蛋白在不同年龄、不同肿瘤大小、不同病理类型、不同临床分期和雌、孕激素受体阴性与阳性患者间的表达均无显著差异(P>0.05)。有淋巴结转移组患者MGMT与无淋巴结转移组患者比较无显著差异(P>0.05)。但GSTπ表达在有淋巴结转移组患者显著高于无淋巴结转移组患者(P<0.05)。相关分析显示,MGMT与GSTπ表达间无显著相关性(P>0.05)。结论初步研究结果提示,MGMT蛋白表达可能与乳腺癌预后无关,不能作为乳腺癌的一个预后指标。GSTπ表达与淋巴结转移状况有关,有可能成为预测乳腺癌预后的指标之一。更多还原

【Abstract】 Objective Our purpose was to explore the clinical significance of MGMT and GST-π protein expression in breast carcinoma tissues and corresponding para-carcinoma tissues.Methods Flow cytometry was used to analyze the expression of MGMT and GST-π protein.Results The expression of MGMT and GST-π were higher in breast carcinoma tissues than that in corresponding para-carcinoma tissues(P< 0.05).There were not notable differences of two drug resistance related protein expressions in different ages,different tumor sizes,different pathological types,different clinical stages,estrogen receptor or progestin receptor negative and positive patients (P> 0.05).There was no notable difference of MGMT expression in lymph node metastasis negative and positive patients(P> 0.05).The expression of GST-π was higher in lymph node metastasis positive patients than that in negative patients(P< 0.05).The correlation analysis showed that there were no correlations between the expressions of MGMT and GST-π(P> 0.05).Conclusion The preliminary research results suggest that MGMT protein expression had probably no correlation with prognosis of breast carcinoma patients,it could not become a prognosis target of breast carcinoma.The expression of GST-π had related with lymph node metastasis status,it can become a target in forecasting breast carcinoma prognosis.更多还原