【摘要】 目的研究直肠癌组织中TAM计数及EMMPRIN、IL-8、MCP-1表达水平并探讨其相互关系和临床病理意义。方法41例直肠癌手术切除标本和15例上切缘非癌粘膜组织常规制作石蜡包埋切片,免疫组化分别染TAM、EMMPRIN、IL-8和MCP-1。结果癌组织TAM计数及EMMPRIN、IL-8、MCP-1表达阳性率明显高于非癌组织;高、中分化腺癌和肿块最大径≤5cm病例,TAM计数明显低于低分化腺癌和肿块最大径>5cm病例;癌细胞未浸及浆膜层病例TAM计数及EMMPRIN、MCP-1表达阳性率明显低于癌细胞浸润浆膜层病例;无淋巴结转移病例TAM计数及EMMPRIN、IL-8、MCP-1表达阳性率明显低于淋巴结转移病例;EMMPRIN、IL-8和MCP-1表达阳性直肠癌TAM计数明显高于阴性病例;直肠癌中EMMPIN、IL-8和MCP-1表达之间存在密切正相关系(P<0.05)。结论TAM计数及EMMPRIN、IL-8和MCP-1表达可能是反映直肠癌发生发展,生物学行为和预后的重要标记物。更多还原

【Abstract】 Objective To study on the TAM counts and the expressions of EMMPRIN, IL-8, MCP-1 and detect their relationships and clinicopathological significances in rectal cancer tissues and noncancer rectal tissues. Methods Lmmunohistochemical method of avidin-biotin complex was used for assays of TAM, EMMPRIN, IL-8 and MCP-1 on the routinely paraffin-embedded sections of surgical resected specimen of 41 cases with rectal cancer and 15 ones with noncancer rectal tissues. Results The TAM counts and the positive rate of EMMPRIN, IL-8 and MCP-1 was significantly higher in rectal cancer tissues than that in noncancer rectal tissues (P< 0.05 or P< 0.01 ). The TAM counts were significantly lower in the cases of well-or middly-differentiation and the maximal diameter of mass ≤5cm than that in the ones of poorly-differentiation and the maximal diameter of mass>5cm (P< 0.01 ). The TAM counts and the positive rates of EMMPRIN and MCP-1 were significantly lower in the cases without infiltrating serous cayer than that in the ones with infiltrating serous layer (P< 0.05 or P< 0.01 ). The TAM counts and the positive rates of EMMPRIN, IL-8 and MCP-1 were significantly lower in without metastasis cases of lymphnode than that in with-metastasis ones of lymphnode (P< 0.05 or P< 0.01 ). The TAM counts were significantly higher in the positive cases of EMMPRIN IL-8 and MCP-1 than that in the negative ones. The positive correlations were found among the expression of EMMPRIN, IL-8 and MCP-1 in rectal cancer tissues. Conclusion TAM count and the expression of EMMPRIN, IL-8 or MCP-1 might be important biological markers for reflecting the carcinogenesis, progression, biological behaviors and prognosis of rectal cancer. The correlative effeets on.更多还原