【摘要】 目的:观察急性应激和/或急性吗啡暴露(2mg/kg)对4周龄和10周龄大鼠记忆的影响。方法:建立平台应激模型,运用Morris水迷宫来观察急性应激和急性吗啡暴露对4周龄和10周龄大鼠空间记忆的影响。结果:①4周龄组:急性应激组、吗啡组及急性应激加吗啡组在目标象限停留的时间均比对照组短(P<0.05)。②10周龄组:急性应激组在目标象限停留的时间比对照组短(P<0.05)。吗啡组及急性应激加吗啡组与对照组相比差异无显著性(P>0.05)。③两个年龄段的比较:10周龄吗啡组、急性应激加吗啡组在目标象限停留的时间均比4周龄要长(P<0.05)。而急性应激组和对照组这两个年龄段分别比较差异无显著性(P>0.05)。两个年龄组中各组大鼠的运动速度分别与对照组相比差异无显著性(P>0.05)。结论:①急性平台应激损害4周龄和10周龄大鼠的记忆,但没有明显年龄差异;②急性吗啡暴露(2mg/kg)对4周龄大鼠的记忆有损害作用,而对10周龄的记忆没有影响,存在年龄差异。③急性应激加吗啡损害了4周龄大鼠的记忆保持,而对10周龄大鼠的记忆保持无影响,存在年龄差异。更多还原

【Abstract】 Objective: To study the effects of acute stress and acute morphine exposure(2mg/kg) on spatial memory in 4-or 10-week-old rats.Methods: Morris water maze was used to assess the effects of acute stress(established by elevated platform stress protocol) and acute morphine exposure on spatial memory in 4-or 10-week-old rats.Results: ① 4-week-old rats: Rats of acute stress group,acute stress and morphine group and morphine group spent significantly less time in the target quadrant than that of control group,respectively(P<0.05).②10-week-old rats: Rats of acute stress group spent significantly less time in the target quadrant than that of control group(P<0.05).Compared with control group time in the target quadrant did not differ in acute stress plus morphine group,morphine group(P >0.05).③Comparison between 10-week-old rats and 4-week-old rats:10-week-old rats in morphine group,acute stress plus morphine group spent significantly more time in the target quadrant than that of 4-week-old rats in the same groups,respectively(P<0.05).Time in the target quadrant did not differ in acute stress group and control group between 4-week-old rats and 10-week-old rats(P >0.05).Swimming speed did not differ between all groups of 4-or 10-week-old rats(P >0.05).Conclusion: ①Acute stress induced impairment of memory retention in 4-or 10-week-old rats,respectively;but it did not differ between ages.②Acute morphine exposure at the dose of 2mg/kg induced impairment of memory retention in 4-week-old rats and had no effect on memory retention in 10-week-old rats.It showed difference between two age groups.③Acute stress plus morphine induced impairment of memory retention in 4-week-old rats and had no effect on memory in 10-week-old rats.It showed difference between two age groups.更多还原