【摘要】 红色糖多孢菌D在15L发酵罐的基础培养基和发酵过程中补加豆油,当基础培养基中豆油量加倍时,效价提高43.1%;发酵过程补加豆油罐的红霉素效价、生产强度和Yp/s比不补豆油的分别提高了69.7%、69.6%和63.8%。通过实验测定发现,发酵过程补加豆油后,异柠檬酸脱氢酶、甲基丙二酰CoA异构酶的比活以及α-酮戊二酸的量均高于不补加豆油时的值,而琥珀酸的量低于不补加豆油时的值。推测豆油经过红色糖多孢菌代谢后进入红霉素合成的一条可能途径:豆油经过代谢后进入TCA循环,并强化了TCA循环的通量,产生了大量α-酮戊二酸,再生成琥珀酰CoA,琥珀酰CoA在甲基丙二酰CoA异构酶的作用下生成甲基丙二酰CoA作为红霉素合成的前体。更多还原

【Abstract】 The final production of erythromycin by Saccharopolyspora erythraea was increased by 43.1% in 15L bioreactor with double quantily of soybean oil into culture medium.The final production,production capacity and Y_p/s of erythromycin were improved 69.7%,69.6% and 63.8%,respectively,in 15L bioreactor with added soybean oil during fermentation.Specific activity of NADP⁺-isocitrate dehydrogenase,methylmalonyl-coenzyme A mutase and the concentration of α-ketoglutaric acid were higher with added（soybean） oil than that not added,and the concentration of succinic acid was lower than that without added（soybean） oil.Therefore,the metabolic pathway from soybean oil to erythromycin was presumed as follows: soybean oil is metabolized into TCA pathway to generate much α-ketoglutaric acid and then generate succinyl-CoA by strengthening TCA pathway,and the methylmalonyl-CoA is formed from succinyl-CoA by isomerization.Finally,methylmalonyl-CoA can be used as the precursor for the biosynthesis of erythromycin.更多还原