【摘要】 以始旋链霉菌(S trep tomy ces p ristinaesp ira lis)HCCB 2003-8的染色体DNA为模板,通过优化PCR条件扩增出FM N还原酶、P IIA合酶的基因片段。与文献报道的基因序列做同源比较,snaA和snaB碱基序列分别只有一个碱基的差异,snaC碱基序列完全匹配。将snaA、snaB和snaC的基因片段插入原核表达载体pET 30a中,并转化至大肠埃希菌BL 21(DE 3)。SDS-PAGE结果表明,经IPTG诱导后基因分别得到表达。更多还原

【Abstract】 Through PCR method and optimization of PCR reaction system,snaA,snaB and snaC gene were obtained from Streptomyces pristinaespiralis HCCB2003-8.Analysis of snaA,snaB and snaC gene(sequences) showed that there was only one base,different from the reported sequences of snaA and snaB respectively.snaC sequence was identical to the reported sequence.snaA,snaB and snaC gene were inserted into pET30a vector and were transformed into E.coli BL21(DE3) strain.After induced by IPTG,the result of SDS-PAGE showed that they were expressed.更多还原