【摘要】 一株来源于海泥的放线菌S1001发酵产物具有致细胞坏死活性,本文采用溶剂萃取、硅胶柱层析及制备HPLC等分离手段对该菌株发酵产物的活性部位进行了活性追踪分离,共分离得到8个化合物,通过理化性质及波谱学手段,鉴定其结构分别为4,′5,7-三羟基异黄酮(1),4-(2,4-二羟基苯酰胺基)苯甲酸(2),环(甘氨酸-丙氨酸)(3),环(甘氨酸-脯氨酸)(4),环(亮氨酸-酪氨酸)(5),环(缬氨酸-亮氨酸)(6),环(缬氨酸-异亮氨酸)(7),环(苯丙氨酸-甘氨酸)(8)。并用SRB法初步评价了化合物1~8的抗肿瘤活性,结果表明化合物4、5和7在10μm o l/L浓度时对K 562细胞表现出了一定的抑制活性。更多还原

【Abstract】 To explore the antitumor active component in fermentation of a marine derived actinomycete S1001,8 compounds were isolated and purified by using solvent extraction,silica gel column and preparative HPLC.The separation procedure was traced by a bioassay using tsFT210 cell lines.By means of physico-chemical properties and spectral analyses,the structures of compounds were determined as: 4′,5,7-trihydroxyisoflavone(),4-(2,4-dihydroxybenzamido) benzoic acid (2),cyclo-(Gly-Ala)(3),cyclo-(Gly-Pro) (4),(cyclo)-(Leu-Tyr)(5),cyclo-(Val-Leu)(6),cyclo-(Val-Ile)(7),cyclo-(Phe-Gly)(8),respectively.The antitumor activities of these compounds were assayed by the SRB method against K562 cell line.Compounds 4, 5 and 7 showed cytotoxicity at the concentration of 10 μmol/L.更多还原