【摘要】 目的观察丰富康复训练对大鼠缺血再灌注脑损伤功能恢复的影响,以及损伤对侧大脑皮层前肢运动代表区第V层锥体神经元树突的可塑性变化。方法雄性Wistar大鼠32只,体重180~200 g,预训练后随机分成两组,缺血组和假手术组,每组16只。线栓法制作右大脑中动脉阻断(MCAO)2 h再灌注模型。造模后缺血组随机分成丰富训练组(IE组)和独居组(IS组),假手术组随机分成丰富训练组(SE组)和独居组(SS组),每组8只。术后24 h,IE组和SE组置于丰富环境笼饲养,按计划给予跑笼、转棒和杂技等训练,IS组和SS组置于独居笼饲养,不给予任何训练,4组大鼠在造模后24 h、1周、2周、3周、4周进行神经功能评估,观察其恢复状况。用Golgi-Cox染色方法,观察损伤对侧大脑皮层前肢运动代表区第V层锥体神经元树突的变化。结果IE组在各项功能评估中均优于IS组。3周时,肢体放置测试IE组与假手术组已无显著性差异(P>0.05);4周时,足失误测试IE组与假手术组已无显著性差异(P>0.05)。Golgi-Cox染色,IE组比IS组和假手术组的树突分支点数有明显增加(P<0.01)。结论丰富康复训练能有效促进缺血再灌注脑损伤大鼠的功能恢复,并促进缺血对侧与功能恢复相关的大脑皮层神经元发生可塑性变化。更多还原

【Abstract】 ObjectiveTo study the effect of enriched rehabilitative training on the functional recovery and neuronal dentritic growth following cerebral ischemia/reperfusion.Methods32 male Wistar rats,weighting 180~200 g,were randomly divided into a ischemic group(n=16) and a sham-operation group(n=16) after beforehand trainings.Rats were subjected to 2 h of right middle cerebral artery occlusion before reperfusion.After surgery,the ischemic group were randomly divided into a ischemia + enrichment(IE) group and a ischemia + standard housing(IS) group;the sham-operation group were randomly divided into a sham + enrichment(SE) group and a sham + standard housing(SS) group.After 24 h reperfusion,IE and SE groups were housed in enriched cages,and given enriched rehabilitative training according to the scheme.At the same time,IS and SS groups were housed in standard cages without any training.The functions of 4 groups were evaluated at 24 h,1 week,2 weeks,3 weeks and 4 week after operation.Dentritic growth of layer V pyramidal cells of the undamaged forelimb motor cortex was examined using Golgi-Cox procedure.ResultsIE group showed better function than IS group in all behavioral test.There was no significant difference in limb-placement test at 3 weeks(P>0.05) and in footfault test at 4 weeks(P>0.05) after operation between IE and SE group.The mean of basilar dentrite branching points in IE group was significantly greater than that of other groups(P<0.01).ConclusionEnriched rehabilitative training can promote functional recovery and enhance neural plasticity after cerebral ischemia/ reperfusion in rats.更多还原